| CASE REPORT |
|
| Year : 2019 | Volume
: 6
| Issue : 4 | Page : 184-187 |
|
|
Identification of docetaxel, cisplatin, and 5-fluorouracil regimen hypersensitivity by In vitro T-cell activation assay
Hang Huong Ling1, Chun-Bing Chen2, Cheng-Hsu Wang1, Yen-Min Huang1
1 Division of Hematology and Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Keelung and Chang Gung University College of Medicine, Taoyuan, Taiwan
2 Department of Dermatology, Drug Hypersensitivity Clinical and Research Center, Chang Gung Memorial Hospital at Keelung and Chang Gung University College of Medicine, Taoyuan, Taiwan
Correspondence Address:
Dr. Yen-Min Huang
Division of Hematology and Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Keelung, No. 200, Lane 208, Jijin 1st Road, Keelung 20445
Taiwan
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/JCRP.JCRP_11_19
|
|
|
An in vitro T-cell activation assay measuring granulysin and granzyme B has been used to identify the drug hypersensitivity of common causative drugs, but not of chemotherapeutic drugs. Both granulysin and granzyme B are cytotoxic molecules involved in skin eruptions during drug hypersensitivity. Herein, we report the first clinical application of an in vitro T-cell activation assay to identify the causative agent in docetaxel, cisplatin, and 5-fluorouracil (5-FU)-related hypersensitivity in a patient with head-and-neck cancer. A significant increase in granulysin and granzyme B was observed for 5-FU rather than for docetaxel or cisplatin. Despite several limitations, we were still able to pinpoint 5-FU as the culprit drug in a chemotherapy combination without further drug rechallenge in our patient. In conclusion, an in vitro T-cell activation assay measuring granulysin and granzyme B can be a safe and alternative tool to determine the causative agent of hypersensitivity reactions in cancer patients who need combination chemotherapy.
|
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
